Imaging, Diagnosis, Prognosis A Prospective PCR-Based Screening for the EML4-ALK Oncogene in Non–Small Cell Lung Cancer
نویسندگان
چکیده
Purpose: EML4-ALK is a lung cancer oncogene, and ALK inhibitors showmarked therapeutic efficacy for tumors harboring this fusion gene. It remains unsettled, however, how the fusion gene should be detected in specimens other than formalin-fixed, paraffin-embedded tissue. We here tested whether reverse transcription PCR (RT-PCR)-based detection of EML4-ALK is a sensitive and reliable approach. Experimental Design:We developed a multiplex RT-PCR system to capture ALK fusion transcripts and applied this technique to our prospective, nationwide cohort of non–small cell lung cancer (NSCLC) in
منابع مشابه
A prospective PCR-based screening for the EML4-ALK oncogene in non-small cell lung cancer.
PURPOSE EML4-ALK is a lung cancer oncogene, and ALK inhibitors show marked therapeutic efficacy for tumors harboring this fusion gene. It remains unsettled, however, how the fusion gene should be detected in specimens other than formalin-fixed, paraffin-embedded tissue. We here tested whether reverse transcription PCR (RT-PCR)-based detection of EML4-ALK is a sensitive and reliable approach. ...
متن کاملA prospective PCR–based screening for the EML4-ALK oncogene in non-small cell lung cancer Running title: PCR–based screening for EML4-ALK
Division of Functional Genomics, Jichi Medical University, Tochigi 329-0498, Japan; Department of Respiratory Medicine, Toho University Omori Medical Center, Tokyo 143-8541, Japan; Tohoku University Hospital, Miyagi 980-8574, Japan; Miyagi Cancer Center, Miyagi 981-1293, Japan; First Department of Medicine, Hokkaido University School of Medicine, Hokkaido 060-8638, Japan; Asahikawa Medical Cent...
متن کاملMultiplex reverse transcription-PCR screening for EML4-ALK fusion transcripts.
PURPOSE EML4-ALK is a fusion-type protein tyrosine kinase that is generated by inv(2)(p21p23) in the genome of non-small cell lung cancer (NSCLC). To allow sensitive detection of EML4-ALK fusion transcripts, we have now developed a multiplex reverse transcription-PCR (RT-PCR) system that captures all in-frame fusions between the two genes. EXPERIMENTAL DESIGN Primers were designed to detect a...
متن کاملEML4-ALK fusion gene and efficacy of an ALK kinase inhibitor in lung cancer.
PURPOSE The EML4-ALK fusion gene has been detected in approximately 7% of Japanese non-small cell lung cancers (NSCLC). We determined the frequency of EML4-ALK in Caucasian NSCLC and in NSCLC cell lines. We also determined whether TAE684, a specific ALK kinase inhibitor, would inhibit the growth of EML4-ALK-containing cell lines in vitro and in vivo. EXPERIMENTAL DESIGN We screened 305 primar...
متن کاملInduction of PD-L1 Expression by the EML4-ALK Oncoprotein and Downstream Signaling Pathways in Non-Small Cell Lung Cancer.
PURPOSE Therapies targeted to the immune checkpoint mediated by PD-1 and PD-L1 show antitumor activity in a subset of patients with non-small cell lung cancer (NSCLC). We have now examined PD-L1 expression and its regulation in NSCLC positive for the EML4-ALK fusion gene. EXPERIMENTAL DESIGN The expression of PD-L1 at the protein and mRNA levels in NSCLC cell lines was examined by flow cytome...
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